TgTn(sb-lacZ,GFP)IR3Jtak
Transgene Detail

Summary

• Symbol: TgTn(sb-lacZ,GFP)IR3Jtak
• Name: transgenic transposon concatemer IR3, Junji Takeda
• MGI ID: MGI:4836983
• Transgene: TgTn(sb-lacZ,GFP)IR3Jtak Location: unknown Genetic Position: Chr12, Syntenic
• Alliance: TgTn(sb-lacZ,GFP)IR3Jtak page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Transposon concatemer)
• Mutation: Insertion
• Mutation details: A concatemer of sleeping beauty transposable elements each consisting of a floxed PGK-neo cassette followed by inverted repeat direct repeat sequence specific for sleeping beauty transposase flanking human BCL2 splice acceptor sequence, IRES, lacZ, poly A signal, a chimeric CAG promoter containing sequence from the chicken ACTB promoter, GFP, and splice donor sequence. Expression of GFP is not seen in the concatemer. Transposition inside a gene should result in ubiquitous expression of GFP by the CAG promoter and expression of lacZ driven by the endogenous promoter. Transposition by sleeping beauty transposase does not move the PGK-neo cassette. The donor mice contain approximately 20 copies of the transposon vector determined by real-time PCR, on distal chromosome 12. (J:104039, J:104043)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:104043 Keng VW, et al., Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system. Nat Methods. 2005 Oct;2(10):763-9
• All: 2 reference(s)