Arid1a^tm1.1Gusa
Targeted Allele Detail

Summary

• Symbol: Arid1a^tm1.1Gusa
• Name: AT-rich interaction domain 1A; targeted mutation 1.1, Guy Sauvageau
• MGI ID: MGI:4836625
• Synonyms: BAF250a^E2E3
• Gene: Arid1a Location: Chr4:133406319-133484080 bp, - strand Genetic Position: Chr4, 66.25 cM
• Alliance: Arid1a^tm1.1Gusa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164526
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2 and a floxed neo cassette was inserted downstream of exon 3. Cre mediated recombination removed exon 2, exon 3, and the neo cassette. The expression of a truncated protein was confirmed by western blot analysis on embryonic fibroblasts. The truncated protein lacks 150 amino acids of the N-terminus without affecting expression levels or protein interaction with Smarca4 (Brg1). (J:164526)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arid1a Mutation: 179 strains or lines available

References

• Original: J:164526 Krosl J, et al., A mutant allele of the Swi/Snf member BAF250a determines the pool size of fetal liver hemopoietic stem cell populations. Blood. 2010 Sep 9;116(10):1678-84
• All: 1 reference(s)