Runx1^{tm2.1(Runx1/Mecom)Homy}
Targeted Allele Detail

Summary

• Symbol: Runx1^{tm2.1(Runx1/Mecom)Homy}
• Name: runt related transcription factor 1; targeted mutation 2.1, Hiroaki Honda
• MGI ID: MGI:4835664
• Synonyms: Runx1^REdeltaIRES
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^{tm2.1(Runx1/Mecom)Homy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164806
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cellular IRES in the 5' UTR of exon 3, the coding region of exon 3 and part of intron 3 were replaced with a floxed neo cassette and a Runx1 and Mecom fusion cDNA via homologous recombination. The neo cassette was removed by cre mediated recombination. RT_PCR confirmed the expression of the fusion gene from both endogenous promoters. Western blot analysis confirmed expression of the fusion protein. (J:164806)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:164806 Nagamachi A, et al., A 5' untranslated region containing the IRES element in the Runx1 gene is required for angiogenesis, hematopoiesis and leukemogenesis in a knock-in mouse model. Dev Biol. 2010 Sep 15;345(2):226-36
• All: 1 reference(s)