Hprt1^{tm44(Ple49-lacZ)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm44(Ple49-lacZ)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 44, Elizabeth M Simpson
• MGI ID: MGI:4834779
• Synonyms: DBH-B-lacZ, Hprt^{tm44(mEMS3682)Ems}, Ple49
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm44(Ple49-lacZ)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS3675, mEMS3682, mEMS3685, mEMS3686
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: mEMS1202 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted
• Mutation: Insertion Vector Type: Not Applicable
• Mutation details: The Ple49-lacZ transgene (pEMS1520) was designed with the 756 bp Ple49 minipromoter (DBH-B; derived from a subsection of the promoter and an upstream putative regulatory element of the human dopamine beta-hydroxylase (dopamine beta-monooxygenase) [DBH] gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

  The promoter/regulatory regions of the human dopamine beta-hydroxylase (DBH) gene directs expression of beta galactosidase (lacZ) to the noradrenergic cells in the locus coeruleus, with strong expression in the adrenal gland where it is coexpressed with tyrosine hydroxylase. (J:144244, J:164356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1274 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS3682 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 3 reference(s)