Gad1tm2(cre)Tama
Targeted Allele Detail
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Symbol: |
Gad1tm2(cre)Tama |
Name: |
glutamate decarboxylase 1; targeted mutation 2, Nobuaki Tamamaki |
MGI ID: |
MGI:4833682 |
Synonyms: |
Gad1tm1(cre)Tama, GAD67-Cre |
Gene: |
Gad1 Location: Chr2:70391644-70432358 bp, + strand Genetic Position: Chr2, 41.63 cM, cytoband D
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Alliance: |
Gad1tm2(cre)Tama page
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Germline Transmission: |
Earliest citation of germline transmission:
J:164349
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
C57BL/6
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Allele Type: |
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Targeted (Recombinase) |
Mutations: |
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Insertion, Intragenic deletion
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Gad1tm2(cre)Tama expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Gad1 (MGI:95632) |
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Mutation details: A targeting vector was designed where the cre recombinase gene was inserted into immediately after the translational initiation site of the Gad1 (GAD67) gene in frame. This vector contained a 3-kb fragment at the 5' side, the cre gene inserted behind the Gad1 translational start, a Pgk-neo-p(A) cassette flanked by two Flp recognition target (FRT) sites, a 7-kb fragment at the 3' side, and an MC1 promoter-driven diphtheria toxin gene for selection. The vector was introduced into C57BL/6 ES cells and ES cells showing homologous recombination were injected into 8-cell stage CD-1 embryos. This allele is a null allele.
(J:164349, J:173688)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Gad1
(mouse)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gad1 Mutation: |
64 strains or lines available
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Original: |
J:164349 Higo S, et al., Subtypes of GABAergic neurons project axons in the neocortex. Front Neuroanat. 2009;3:25 |
All: |
11 reference(s) |
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