Serpina6^tm1.1Mmp
Targeted Allele Detail

Summary

• Symbol: Serpina6^tm1.1Mmp
• Name: serine (or cysteine) peptidase inhibitor, clade A, member 6; targeted mutation 1.1, Marie-Pierre Moisan
• MGI ID: MGI:4833641
• Synonyms: Cbg^-, Cbg knockout, transcortin knockout
• Gene: Serpina6 Location: Chr12:103612889-103623471 bp, - strand Genetic Position: Chr12, 52.98 cM
• Alliance: Serpina6^tm1.1Mmp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164345
• Parent Cell Line: MCI (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The loxP-flanked exon 2 of Serpina6^tm1Mmp, containing the entire protein coding sequence, has been excised by Cre recombinase. Quantitative real-time PCR analysis of liver mRNA detects no transcripts from the targeted gene in homozygous mutant mice and ~50% of wild-type transcript levels in heterozygous mice. Immunoblot analysis of plasma of homozygous mutant, heterozygous and wild-type mice yields confirmatory results for the for the protein. (J:164345)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Serpina6 Mutation: 35 strains or lines available

Notes

Gene targeting to generate Serpina6^tm1Mmp was performed at the Mouse Clinical Institute (Illkirch, France)(J:164345), which according to their Web site (http://www.ics-mci.fr/index.html) employs "ES cells of the 129SvPas strain."

References

• Original: J:164345 Richard EM, et al., Plasma transcortin influences endocrine and behavioral stress responses in mice. Endocrinology. 2010 Feb;151(2):649-59
• All: 3 reference(s)