Comt^tm1Mkae
Targeted Allele Detail

Summary

• Symbol: Comt^tm1Mkae
• Name: catechol-O-methyltransferase; targeted mutation 1, Mikko Kaenmaki
• MGI ID: MGI:4830700
• Synonyms: S-COMT
• Gene: Comt Location: Chr16:18225636-18245602 bp, - strand Genetic Position: Chr16, 11.4 cM
• Alliance: Comt^tm1Mkae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164057
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A mutation of the methionine coding start codon (ATG) for S-COMT to threonine (ACC), corresponding to 'ATG' position +30 in exon2 of Comt gene and a floxed neo cassette in intron 4 were introduced by homologous recombination. The selection cassette was removed by cre mediated recombination. The S-COMT (24 kDa) isoform was absent on western blots of liver extracts, while the MB-COMT was detected. (J:164057)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Comt Mutation: 25 strains or lines available

Notes

ES cell line = 3B4

References

• Original: J:164057 Kaenmaki M, et al., Importance of membrane-bound catechol-O-methyltransferase in L-DOPA metabolism: a pharmacokinetic study in two types of Comt gene modified mice. Br J Pharmacol. 2009 Dec;158(8):1884-94
• All: 2 reference(s)