Tg(RP23-291P1)#Flp
Transgene Detail

Summary

• Symbol: Tg(RP23-291P1)#Flp
• Name: transgene insertion, Figge, Lammert and Paigen
• MGI ID: MGI:4822036
• Synonyms: BSEP OE, TTR-Abcb11
• Transgene: Tg(RP23-291P1)#Flp Location: unknown
• Alliance: Tg(RP23-291P1)#Flp page

Transgene origin

• Strain of Origin: 129S1/SvImJ

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(RP23-291P1)#Flp expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Abcb11 (MGI:1351619)
  mouse	G6pc2 (MGI:1277193)

• Mutation details: The transgene comprises one to two copies of bacterial artificial chromosome (BAC) clone RP23-291P1, whose 182-kb, C57BL/6J-derived genomic DNA insert contains the Abc11 gene, 33 kb of 5' flanking and 44 kb of 3' flanking DNA, and the entire glucose-6-phosphatase, catalytic, 2 (G6pc2) gene. Northern blot analysis of total liver RNA and western blot analysis of canalicular liver plasma membrane extracts showed about two-fold elevation of Abc11 transcript and protein levels in transgenic mice fed a chow diet; transcript levels were unaffected by consumption of a lithogenic diet for 8 weeks. (J:94496)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:163323 Halpern MD, et al., Apical sodium-dependent bile acid transporter upregulation is associated with necrotizing enterocolitis. Am J Physiol Gastrointest Liver Physiol. 2010 Sep;299(3):G623-31
• All: 2 reference(s)