Clec1b^tm1Mlkn
Targeted Allele Detail

Summary

• Symbol: Clec1b^tm1Mlkn
• Name: C-type lectin domain family 1, member b; targeted mutation 1, Mark L Kahn
• MGI ID: MGI:4820842
• Synonyms: Clec-2^-
• Gene: Clec1b Location: Chr6:129374260-129382376 bp, + strand Genetic Position: Chr6, 63.3 cM, cytoband F3
• Alliance: Clec1b^tm1Mlkn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:162815
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo cassette replaced the promoter and exon 1 (which encodes the entire intracellular domain of the Clec1b receptor, including the YxxL motif required to activate Syk, as well as the transcriptional and translational start sites.) Immunoblotting of cell lysate from platelet-rich plasma of heterozygous animals showed that Clec1b protein levels were about 50% of wild-type and levels were undetectable in homozygous mutants. (J:162815)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clec1b Mutation: 17 strains or lines available

References

• Original: J:162815 Bertozzi CC, et al., Platelets regulate lymphatic vascular development through CLEC-2-SLP-76 signaling. Blood. 2010 Jul 29;116(4):661-70
• All: 7 reference(s)