Gsk3b^{tm2a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gsk3b^{tm2a(EUCOMM)Wtsi}
• Name: glycogen synthase kinase 3 beta; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4820225
• Gene: Gsk3b Location: Chr16:37909363-38066446 bp, + strand Genetic Position: Chr16, 26.54 cM, cytoband B4
• IMPC: Gsk3b gene page

Mutation origin

• Mutant Cell Lines: EPD0615_2_A09, EPD0615_2_A10, EPD0615_2_A11, EPD0615_2_C07, EPD0615_2_C08, EPD0615_2_C10, EPD0615_2_D07, EPD0615_2_F07, EPD0615_2_F09, EPD0615_2_G07, EPD0615_2_G08, EPD0615_2_G10, EPD0615_2_H08, EPD0615_2_H10, EPD0615_2_H11
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 38007683 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 38008547. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Gsk3b Mutation: 111 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)