Cyld^tm1.1Gmos
Targeted Allele Detail

Summary

• Symbol: Cyld^tm1.1Gmos
• Name: CYLD lysine 63 deubiquitinase; targeted mutation 1.1, George Mosialos
• MGI ID: MGI:4819959
• Synonyms: Cyld^delta9
• Gene: Cyld Location: Chr8:89423675-89478573 bp, + strand Genetic Position: Chr8, 43.51 cM
• Alliance: Cyld^tm1.1Gmos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:162840
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette was inserted upstream of exon 9 and an additional loxP site was inserted downstream of exon 9. Cre mediated recombination removed the neo cassette and exon 9. This exon codes for an essential catalytic motif of the deubiquitination domain. The absence of protein expression was confirmed by western blot analysis on fibroblast extracts. (J:162840)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyld Mutation: 43 strains or lines available

References

• Original: J:162840 Trompouki E, et al., Truncation of the catalytic domain of the cylindromatosis tumor suppressor impairs lung maturation. Neoplasia. 2009 May;11(5):469-76
• All: 3 reference(s)