Pms2^tm1.1Wed
Targeted Allele Detail

Summary

• Symbol: Pms2^tm1.1Wed
• Name: PMS1 homolog2, mismatch repair system component; targeted mutation 1.1, Winfried Edelmann
• MGI ID: MGI:4819008
• Synonyms: Pms2^E702K, Pms2^EK
• Gene: Pms2 Location: Chr5:143846782-143870786 bp, + strand Genetic Position: Chr5, 82.82 cM, cytoband G2
• Alliance: Pms2^tm1.1Wed page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:162378
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An E702K mutation was introduced into exon 12 disrupting the DQHA(X)2E(X)4E metal binding motif. In addition, a floxed hygro/neo selection cassette was introduced in intron 12. Cre mediated recombination then removed the selection cassette. RT-PCR and qPCR indicted that expression levels of the mutant allele were similar to wild-type in MEFs and primary B cells. Western blot analysis indicated about a 25% reduction in protein levels in the spleens, brains, and testes of homozygous mice compared to wild-type tissues. (J:162378)

Tumor Data

List all tumor models in MMHCdb carrying Pms2^tm1.1Wed

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pms2 Mutation: 50 strains or lines available

References

• Original: J:162378 van Oers JM, et al., PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13384-9
• All: 1 reference(s)