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Adam23tm1a(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Adam23tm1a(EUCOMM)Hmgu
Name: a disintegrin and metallopeptidase domain 23; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4462649
Gene: Adam23  Location: Chr1:63484880-63635263 bp, + strand  Genetic Position: Chr1, 32.31 cM
Alliance: Adam23tm1a(EUCOMM)Hmgu page
IMPC: Adam23 gene page
Mutation
origin
Mutant Cell Lines:  HEPD0658_5_A05, HEPD0658_5_A06, HEPD0658_5_A07, HEPD0658_5_B05, HEPD0658_5_B06, HEPD0658_5_B07, HEPD0658_5_B08, HEPD0658_5_C05, HEPD0658_5_C06, HEPD0658_5_C08, HEPD0658_5_D07, HEPD0658_5_D08, HEPD0658_5_E05, HEPD0658_5_E06, HEPD0658_5_F08, HEPD0658_5_G06, HEPD0658_5_G08, HEPD0658_5_H06, HEPD0658_5_H07, HEPD0658_5_H08
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 63573023 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 63573749. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 1 line available
Carrying any Adam23 Mutation:  62 strains or lines available
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory