Cilk1^{tm1a(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Cilk1^{tm1a(KOMP)Mbp}
• Name: ciliogenesis associated kinase 1; targeted mutation 1a, Mouse Biology Program, UC Davis
• MGI ID: MGI:4462529
• Synonyms: Ick^tm1a
• Gene: Cilk1 Location: Chr9:78016474-78079389 bp, + strand Genetic Position: Chr9, 43.56 cM, cytoband E1
• Alliance: Cilk1^{tm1a(KOMP)Mbp} page
• IMPC: Cilk1 gene page

Endocrine-cerebro-osteodysplasia syndrome-like features in Cilk1^{tm1a(KOMP)Mbp}/Cilk1^{tm1a(KOMP)Mbp} mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: DEPD00524_1_A09, DEPD00524_1_A10, DEPD00524_1_A11, DEPD00524_1_B09, DEPD00524_1_C09, DEPD00524_1_C10, DEPD00524_1_C11, DEPD00524_1_C12, DEPD00524_1_D09, DEPD00524_1_D11, DEPD00524_1_D12, DEPD00524_1_E09, DEPD00524_1_E12, DEPD00524_1_F09, DEPD00524_1_F10, DEPD00524_1_F11, DEPD00524_1_F12, DEPD00524_1_G09, DEPD00524_1_G10, DEPD00524_1_G12, DEPD00524_1_H09, DEPD00524_1_H10, DEPD00524_1_H11, DEPD00524_1_H12
• Germline Transmission: Earliest citation of germline transmission: J:211652
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 78057267 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 78058201. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Cilk1 Mutation: 52 strains or lines available

Notes

The mice reported in J:211652 were created from the DEPD00524_1_A09 mutant cell line.

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 9 reference(s)