Tg(Per2-DsRed*T3)12Obr
Transgene Detail

Summary

• Symbol: Tg(Per2-DsRed*T3)12Obr
• Name: transgene insertion 12, Karl Obrietan
• MGI ID: MGI:4456978
• Synonyms: mPeriod2-DsRed
• Transgene: Tg(Per2-DsRed*T3)12Obr Location: unknown
• Alliance: Tg(Per2-DsRed*T3)12Obr page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene comprises bacterial artificial chromosome (BAC) #RP24-391L2 containing the mouse period 2 (Per2) gene into which a DsRed.T3-NLS-PEST fusion gene has been inserted. The DsRed.T3 variant contains eight point mutations relative to the parent DsRed gene that increase the protein's maturation kinetics ~10-fold. To improve the protein's kinetic properties further, the PEST domain of the mouse ornithine decarboxylase gene was introduced following the penultimate codon of the gene. A nuclear localization signal (NLS) was cloned in-frame between the DsRed coding sequence and the PEST domain. The sequence encoding the fusion protein is preceded by a human cytomegalovirus (hCMV) promoter and followed by an SV40 polyadenylation signal. Its robust expression in neuronal cells of the suprachiasmatic nuclei (SCN) of the hypothalamus is cyclic and light-inducible; it is also expressed at lower levels in glial cells of brain regions outside the SCN. (J:150729)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Three Tg(Per2-DsRed*T3) transgenic lines, nos. 6,8 and 12, exhibit identical patterns of expression in the SCN and other brain regions, differing only in their fluorescence intensity. Data from all three were pooled for qualitative analyses; line 12, which demonstrates the highest expression, was used for quantitative studies. (J:150729)

References

• Original: J:150729 Cheng HY, et al., Segregation of expression of mPeriod gene homologs in neurons and glia: possible divergent roles of mPeriod1 and mPeriod2 in the brain. Hum Mol Genet. 2009 Aug 15;18(16):3110-24
• All: 2 reference(s)