Tg(Thy1-COP4*H134R/EYFP)20Gfng
Transgene Detail

Summary

• Symbol: Tg(Thy1-COP4*H134R/EYFP)20Gfng
• Name: transgene insertion 20, Guoping Feng
• MGI ID: MGI:4454792
• Synonyms: Thy1-mhChR2-EYFP Line 20, Thy1-mhChR2-YFP Line 20
• Transgene: Tg(Thy1-COP4*H134R/EYFP)20Gfng Location: unknown
• Alliance: Tg(Thy1-COP4*H134R/EYFP)20Gfng page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A channelrhodopsin-2 cDNA sequence derived from green alga Chlamydomonas reinhardtii was modified for optimal mammalian expression (beta2 nictinic acytlcholine receptor signal peptide (MAGHSNSMALFSFSLLWLCSGVLGTEF) at the N-terminus, and ER-exporting motif (RSRFVKKDGHCNVQFINVGEKGQRYLA) and Golgi-exporting motif (NSFCYENEVALTSK) (both from potassium channel Kir2.1 gene) at the C-terminus) and modified with a gain-of-function H134R substitution (CAC to CGC) designed to cause larger stationary photocurrents. This B2SP-mhChR2-ER-Golgi sequence (also called B2SP-hChR2-H134R-ER export-Golgi export) was fused in-frame to the amino terminus of an enhanced yellow fluorescent protein sequence (EYFP) via a Not1 site with GCGGCCGCC linker sequence. The resulting mhChR2::YFP fusion protein (also called hChR2-H134R-EYFP) was placed downstream of the modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns), and followed by a bovine growth hormone polyA signal. Mice derived from founder line 20 exhibit high EGFP expression in cortex, CA1, thalamus, superior colliculus, inferior culliculus, brainstem, amygdala and cerebellum. (J:160660)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:160660 Feng G, DDS for Tg(Thy1-COP4*H134R/EYFP)20Gfng. MGI Direct Data Submission. 2010
• All: 4 reference(s)