Tg(Thy1-hop/EYFP)2Gfng
Transgene Detail
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Symbol: |
Tg(Thy1-hop/EYFP)2Gfng |
Name: |
transgene insertion 2, Guoping Feng |
MGI ID: |
MGI:4454787 |
Synonyms: |
Thy1-enPHR-ERD Line 2, Thy1-eNpHR-YFP line 2 |
Transgene: |
Tg(Thy1-hop/EYFP)2Gfng Location: unknown
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Alliance: |
Tg(Thy1-hop/EYFP)2Gfng page
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Transgene Type: |
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Transgenic (Reporter) |
Mutation: |
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Insertion
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Mutation details: A fusion protein was created with the halophilic bacterium Natronobacterium pharaonisfunctions halorhodopsin (NpHR) fused in-frame with an enhanced yellow fluorescent protein (EYFP). The fusion protein was optimized for expression in mammalian systems and enhanced membrane translocation/reduced bleb formation via C-terminal addition of an endoplasmic reticulum exporting sequence (amino acids 373 to 385 from the potassium channel Kir2.1 gene [NANSFCYENEVALTSK]). This enhanced fusion protein (eNpHR::YFP) was placed downstream of the modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns), and followed by a bovine growth hormone polyA signal. Mice derived from founder line 2 exhibit high expression of EYFP in layer 5 pyramidal neurons at cortex, dentate gyrus, thalamus, superior colliculus, inferior culliculus, brainstem, amydagala and cerebellum.
(J:160661)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:160661 Feng G, DDS for Tg(Thy1-HOP/EYFP)2Gfng. MGI Direct Data Submission. 2010; |
All: |
3 reference(s) |
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