Cldn16^tm1.1Dmu
Targeted Allele Detail

Summary

• Symbol: Cldn16^tm1.1Dmu
• Name: claudin 16; targeted mutation 1.1, Dominik Muller
• MGI ID: MGI:4453303
• Synonyms: Cldn16^-
• Gene: Cldn16 Location: Chr16:26281885-26301515 bp, + strand Genetic Position: Chr16, 18.1 cM, cytoband B2
• Alliance: Cldn16^tm1.1Dmu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159706
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT/loxP-flanked pgk-neo cassette and an additional loxP sequence were inserted to flank exon 2, which allowed subsequent deletion of the exon as well as the neomycin resistance cassette. Elimination of exon 2 leads to a frameshift and early stop codon and truncation of the protein. The deletion of exon 2 as well as the neomycin resistance gene was accomplished via Cre-mediated recombination. Western blot analysis showed the absence of protein expression in kidney tissue from homozygous mice. (J:159706)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cldn16 Mutation: 14 strains or lines available

References

• Original: J:159706 Will C, et al., Targeted deletion of murine Cldn16 identifies extra- and intrarenal compensatory mechanisms of Ca2+ and Mg2+ wasting. Am J Physiol Renal Physiol. 2010 May;298(5):F1152-61
• All: 2 reference(s)