Cyp11a1^{tm1(GFP/cre)Pzg}
Targeted Allele Detail

Summary

• Symbol: Cyp11a1^{tm1(GFP/cre)Pzg}
• Name: cytochrome P450, family 11, subfamily a, polypeptide 1; targeted mutation 1, Pumin Zhang
• MGI ID: MGI:4442187
• Synonyms: Cyp11a1-GFP-Cre, Cyp11a1^{tm1(GFP/cre)Zhang}
• Gene: Cyp11a1 Location: Chr9:57913694-57934306 bp, + strand Genetic Position: Chr9, 31.63 cM
• Alliance: Cyp11a1^{tm1(GFP/cre)Pzg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159099
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Cyp11a1^{tm1(GFP/cre)Pzg} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cyp11a1 (MGI:88582)

• Mutation details: A targeting vector containing a GFP-Cre cassette (GC) and a FRT site-flanked kanamycin and neomycin resistance cassette was inserted into the first coding exon of the endogenous gene immediately following the ATG translation initiation codon. Although no green fluorescence is visible in whole heterozygous mutant E15.5 embryos or their urogenital tracts, immunohistochemical analysis detects GFP protein in their kidneys and testes. In E15.5 embryos bearing this allele and a Cre recombinase-inducible lacZ reporter gene, beta-galactosidase activity is detected by X-gal staining in kidneys and adrenals of both sexes and in testes. (J:159099)
• Inheritance: Not Specified

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cyp11a1 (mouse) Summary of all recombinase alleles driven by Cyp11a1. Comparative matrix view of recombinase activities Alliance mouse Cyp11a1 gene page

Expression

In Mice Carrying this Mutation:

9 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyp11a1 Mutation: 41 strains or lines available

References

• Original: J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010
• All: 7 reference(s)