Timmdc1tm1a(EUCOMM)Wtsi
Targeted Allele Detail
|
Symbol: |
Timmdc1tm1a(EUCOMM)Wtsi |
Name: |
translocase of inner mitochondrial membrane domain containing 1; targeted mutation 1a, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:4441702 |
Gene: |
Timmdc1 Location: Chr16:38318709-38343025 bp, - strand Genetic Position: Chr16, 26.86 cM
|
Alliance: |
Timmdc1tm1a(EUCOMM)Wtsi page
|
IMPC: |
Timmdc1 gene page |
|
Mutant Cell Lines: |
EPD0496_1_A10, EPD0496_1_A11, EPD0496_1_A12, EPD0496_1_B12, EPD0496_1_C09, EPD0496_1_C11, EPD0496_1_C12, EPD0496_1_D12, EPD0496_1_E12, EPD0496_1_G12, EPD0496_1_H10, EPD0496_1_H12 |
Germline Transmission: |
Earliest citation of germline transmission:
J:204812
|
Parent Cell Line: |
JM8A3.N1 (ES Cell)
|
Strain of Origin: |
C57BL/6N-Atm1Brd
|
Project Collection: |
EUCOMM
|
|
Allele Type: |
|
Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
|
Insertion
Vector: L1L2_Bact_P
|
|
|
Mutation details: The L1L2_Bact_P cassette was inserted at position 38338508 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 38339356. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:155845, J:173534)
|
|
|
|
Original: |
J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010; |
All: |
3 reference(s) |
|