Flt4^tm1.1Ichi
Targeted Allele Detail

Summary

• Symbol: Flt4^tm1.1Ichi
• Name: FMS-like tyrosine kinase 4; targeted mutation 1.1, Hirotake Ichise
• MGI ID: MGI:4441407
• Synonyms: Vegfr3-EGFP
• Gene: Flt4 Location: Chr11:49500506-49543566 bp, + strand Genetic Position: Chr11, 29.69 cM, cytoband A5-B1
• Alliance: Flt4^tm1.1Ichi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159024
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6J x 129S/SvEv)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A vector composed a 5' fragment of Flt4, an EGFP cDNA with a Pgk polyA signal sequence followed by an FRT-flanked Pgk-gb2-neo cassette ligated upstream of the first ATG of the Flt4 gene, and a 3' homology arm encoding an intronic region between exons 1 and 2 of Flt4 was used for targeting the Flt4 locus in (C57BL/6J x 129/SvEv)F1 ES cells. Exon 1 was replaced by EGFP after homologous recombination. The neo cassette was deleted in heterozygous mice by crossing with FLP66 deleter mice. No Flt4 protein is detected by Western blot in homozygous embryos. (J:159024)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Flt4 Mutation: 76 strains or lines available

References

• Original: J:159024 Ichise T, et al., H-, N- and Kras cooperatively regulate lymphatic vessel growth by modulating VEGFR3 expression in lymphatic endothelial cells in mice. Development. 2010 Mar;137(6):1003-13
• All: 4 reference(s)