Clic1^tm1Breit
Targeted Allele Detail

Summary

• Symbol: Clic1^tm1Breit
• Name: chloride intracellular channel 1; targeted mutation 1, Samuel Breit
• MGI ID: MGI:4440468
• Synonyms: Clic1^FN
• Gene: Clic1 Location: Chr17:35269219-35277695 bp, + strand Genetic Position: Chr17, 18.58 cM
• Alliance: Clic1^tm1Breit page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158394
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: An N-terminal FLAG tag was added after the start codon within exon 2 and a single loxP site was inserted in intron 1 within the 5' UTR. A loxP flanked neo was inserted within intron 5. Expression level of flag tagged protein is low. Northern blot analysis revealed that there were multiple transcripts present with a significant reduction of the mature transcript. (J:158394)

Generation of the Clic1^tm1Breit and Clic1^tm1.1Breit alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clic1 Mutation: 17 strains or lines available

References

• Original: J:158394 Qiu MR, et al., Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene. Genesis. 2010 Feb;48(2):127-36
• All: 1 reference(s)