Pvalbtm1.1(tTA2)Hze
Targeted Allele Detail
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| Symbol: |
Pvalbtm1.1(tTA2)Hze |
| Name: |
parvalbumin; targeted mutation 1.1, Hongkui Zeng |
| MGI ID: |
MGI:4439154 |
| Synonyms: |
Pvalb-2A-tTA2 delta hygro |
| Gene: |
Pvalb Location: Chr15:78075314-78090600 bp, - strand Genetic Position: Chr15, 36.93 cM, cytoband E
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| Alliance: |
Pvalbtm1.1(tTA2)Hze page
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| Allele Type: |
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Targeted (Transactivator) |
| Mutation: |
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Insertion
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Mutation details: A targeting vector was designed to insert a viral 2A oligopeptide that mediates ribosomal skipping and a modified tetracycline-regulated transactivator (tTA2) gene immediately downstream of the parvalbumin translational STOP codon. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site, a viral 2A oligopeptide, a synthetic modified tetracycline-regulated transactivator (tTA2S) gene, a translational STOP sequence (TAA codon), an attB site, a PGK-hygromycin-polyA cassette (with an RNA spice donor-frt5 site-RNA spice acceptor in the hygromycin gene), and an attP site. PhiC31 mediated recombination removed the PGK-hygromycin-polyA cassette. Pvalb-2A-tTA2 activates tetO-regulated gene expression in scattered interneuronal populations in the cortex and hippocampus, as well as in neuronal populations in other brain regions, in a pattern that resembles Pvalb expression. Additional reporter gene expression is seen in cortical layer 5 neurons, which are unlikely to be interneurons.
(J:158563)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Pvalb Mutation: |
30 strains or lines available
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| Original: |
J:158563 Zeng H, Direct Data Submission of Pva1b tm1.1(tTA2)Hze. MGI Direct Data Submission. 2010; |
| All: |
1 reference(s) |
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Analysis Tools
