Mrgprd^{tm4.1(COP4)Mjz}
Targeted Allele Detail

Summary

• Symbol: Mrgprd^{tm4.1(COP4)Mjz}
• Name: MAS-related GPR, member D; targeted mutation 4.1, Mark J Zylka
• MGI ID: MGI:4439026
• Synonyms: Mrgprd-ChR2-Venus, Mrgprddelta^{hChR2(H134R)Venus}
• Gene: Mrgprd Location: Chr7:144868572-144877796 bp, + strand Genetic Position: Chr7, 89.06 cM
• Alliance: Mrgprd^{tm4.1(COP4)Mjz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158198
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The genomic region coding for amino acids 2 through 315 of 321 amino acids was replaced with an inframe human cDNA with the nucleotide substitution resulting in the amino acid substitution of arginine for histidine at position 134 (H134R) fused to Venus and followed by a floxed self-excising neo cassette. Expression of the fusion protein in nonpeptidergic neurons was confirmed by immunohistochemistry. (J:158198)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mrgprd Mutation: 24 strains or lines available

References

• Original: J:158198 Wang H, et al., Mrgprd-expressing polymodal nociceptive neurons innervate most known classes of substantia gelatinosa neurons. J Neurosci. 2009 Oct 21;29(42):13202-9
• All: 3 reference(s)