Aicda^{tm1.1(cre/ERT2)Crey}
Targeted Allele Detail

Summary

• Symbol: Aicda^{tm1.1(cre/ERT2)Crey}
• Name: activation-induced cytidine deaminase; targeted mutation 1.1, Claude-Agnes Reynaud
• MGI ID: MGI:4437052
• Synonyms: AID-Cre-ERT2
• Gene: Aicda Location: Chr6:122530768-122541139 bp, + strand Genetic Position: Chr6, 57.65 cM, cytoband F2
• Alliance: Aicda^{tm1.1(cre/ERT2)Crey} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157741
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Aicda^{tm1.1(cre/ERT2)Crey} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Aicda (MGI:1342279)

• Mutation details: A targeting construct was designed to insert a cre/ERT2 sequence, an SV40 polyA site, and a floxed neo selection cassette into the Aicda locus, replacing exon 2 and fusing with sequence encoding the first 4 amino acids, including 4 bases downstream of the exon 2 splice site. The splice acceptor site of Aicda intron 2-3 was mutated to prevent splicing events that could eliminate the cre/ERT2 sequence. E14.1 ES cells were electroporated with the linearized vector. Recombinant clones were transiently transfected with a cre-expressing vector to excise the neo cassette. (J:157741)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Aicda (mouse) Summary of all recombinase alleles driven by Aicda. Alliance mouse Aicda gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Aicda Mutation: 55 strains or lines available

References

• Original: J:157741 Dogan I, et al., Multiple layers of B cell memory with different effector functions. Nat Immunol. 2009 Dec;10(12):1292-9
• All: 20 reference(s)