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Aicdatm1.1(cre/ERT2)Crey
Targeted Allele Detail
Summary
Symbol: Aicdatm1.1(cre/ERT2)Crey
Name: activation-induced cytidine deaminase; targeted mutation 1.1, Claude-Agnes Reynaud
MGI ID: MGI:4437052
Synonyms: AID-Cre-ERT2
Gene: Aicda  Location: Chr6:122530768-122541139 bp, + strand  Genetic Position: Chr6, 57.65 cM, cytoband F2
Alliance: Aicdatm1.1(cre/ERT2)Crey page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:157741
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Inducible, Null/knockout, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
 
Aicdatm1.1(cre/ERT2)Crey expression driven by 1 gene
 
Mutation detailsA targeting construct was designed to insert a cre/ERT2 sequence, an SV40 polyA site, and a floxed neo selection cassette into the Aicda locus, replacing exon 2 and fusing with sequence encoding the first 4 amino acids, including 4 bases downstream of the exon 2 splice site. The splice acceptor site of Aicda intron 2-3 was mutated to prevent splicing events that could eliminate the cre/ERT2 sequence. E14.1 ES cells were electroporated with the linearized vector. Recombinant clones were transiently transfected with a cre-expressing vector to excise the neo cassette. (J:157741)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Aicda (mouse)
Summary of all recombinase alleles driven by Aicda.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Aicda Mutation:  55 strains or lines available
References
Original:  J:157741 Dogan I, et al., Multiple layers of B cell memory with different effector functions. Nat Immunol. 2009 Dec;10(12):1292-9
All:  20 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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last database update
07/05/2024
MGI 6.24
The Jackson Laboratory