Dock10^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Dock10^{tm1a(EUCOMM)Hmgu}
• Name: dedicator of cytokinesis 10; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4436497
• Synonyms: Dock10 KO, Dock10^lacZ, Ziz3 KO
• Gene: Dock10 Location: Chr1:80478790-80736244 bp, - strand Genetic Position: Chr1, 41.41 cM
• Alliance: Dock10^{tm1a(EUCOMM)Hmgu} page
• IMPC: Dock10 gene page

Mutation origin

• Mutant Cell Lines: HEPD0551_3_A09, HEPD0551_3_C11
• Germline Transmission: Earliest citation of germline transmission: J:236911
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 80592234 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 80593538. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:236911)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Dock10 Mutation: 140 strains or lines available

Notes

Cell line HEPD0551_3_C11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:236911

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 8 reference(s)