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Targeted Allele Detail
Symbol: Ube3btm1a(EUCOMM)Hmgu
Name: ubiquitin protein ligase E3B; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4434627
Gene: Ube3b  Location: Chr5:114518668-114559230 bp, + strand  Genetic Position: Chr5, 55.99 cM
Alliance: Ube3btm1a(EUCOMM)Hmgu page
IMPC: Ube3b gene page
Growth retardation in Ube3btm1a(EUCOMM)Hmgu/Ube3btm1a(EUCOMM)Hmgu mice

Show the 4 phenotype image(s) involving this allele.

Mutant Cell Lines:  HEPD0074_4_A09, HEPD0074_4_B07, HEPD0074_4_B09, HEPD0074_4_C07, HEPD0074_4_C08, HEPD0074_4_C09, HEPD0074_4_D07, HEPD0074_4_D09, HEPD0074_4_E07, HEPD0074_4_E08, HEPD0074_4_E09, HEPD0074_4_F07, HEPD0074_4_F08, HEPD0074_4_F09, HEPD0074_4_G07, HEPD0074_4_G08, HEPD0074_4_G09, HEPD0074_4_H07, HEPD0074_4_H08, HEPD0074_4_H09
Germline Transmission:  Earliest citation of germline transmission: J:273879
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt0
Mutation detailsThe L1L2_gt0 cassette was inserted at position 114527986 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 114528771. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:273879)
Schematic of the Ube3btm1a(EUCOMM)Hmgu allele
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 4 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Ube3b Mutation:  44 strains or lines available
Cell line HEPD0074_4_C07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:273879.
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.22
The Jackson Laboratory