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Furintm1a(EUCOMM)Wtsi
Targeted Allele Detail
Summary
Symbol: Furintm1a(EUCOMM)Wtsi
Name: furin, paired basic amino acid cleaving enzyme; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4434157
Gene: Furin  Location: Chr7:80038942-80055188 bp, - strand  Genetic Position: Chr7, 45.65 cM, cytoband D1-E2
Alliance: Furintm1a(EUCOMM)Wtsi page
IMPC: Furin gene page
Mutation
origin
Mutant Cell Lines:  EPD0127_1_A12, EPD0127_1_C12, EPD0127_1_D09, EPD0127_1_E09, EPD0127_1_E10
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 80046476 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 80047378. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Furin Mutation:  45 strains or lines available
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
07/02/2024
MGI 6.13
The Jackson Laboratory