Corttm1(cre)Zjh
Targeted Allele Detail
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Symbol: |
Corttm1(cre)Zjh |
Name: |
cortistatin; targeted mutation 1, Z Josh Huang |
MGI ID: |
MGI:4431363 |
Synonyms: |
Cst-2A-Cre |
Gene: |
Cort Location: Chr4:149209491-149211220 bp, - strand Genetic Position: Chr4, 78.89 cM
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Alliance: |
Corttm1(cre)Zjh page
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Germline Transmission: |
Earliest citation of germline transmission:
J:151755
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Parent Cell Line: |
v6.5 (ES Cell)
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Strain of Origin: |
(C57BL/6 x 129S4/SvJae)F1
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Project Collection: |
Neuroscience Blueprint cre
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Allele Type: |
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Targeted (Recombinase) |
Mutation: |
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Insertion
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Corttm1(cre)Zjh expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), a cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cortistatin locus (Cort). This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to generate the colony and remove the neo selection cassette. The FLPe transgene has been bred out of the line.
(J:151755)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Cort
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009; |
All: |
12 reference(s) |
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