Mcam^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mcam^{tm1(KOMP)Wtsi}
• Name: melanoma cell adhesion molecule; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419884
• Gene: Mcam Location: Chr9:44045958-44054024 bp, + strand Genetic Position: Chr9, 24.68 cM
• Alliance: Mcam^{tm1(KOMP)Wtsi} page
• IMPC: Mcam gene page

Mutation origin

• Mutant Cell Lines: EPD0375_4_A10, EPD0375_4_A12, EPD0375_4_E09, EPD0375_4_E11, EPD0375_4_F09, EPD0375_4_F12
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 4537 starting at position 44047790 and ending at position 44052327 of Chromosome 9 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Mcam Mutation: 38 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)