Clptm1^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Clptm1^{tm1a(KOMP)Wtsi}
• Name: cleft lip and palate associated transmembrane protein 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419487
• Synonyms: Clptm1^{tm1a(KOMP)Wtsi} "KO-first", KOfirst
• Gene: Clptm1 Location: Chr7:19365505-19398955 bp, - strand Genetic Position: Chr7, 9.93 cM, cytoband A2
• Alliance: Clptm1^{tm1a(KOMP)Wtsi} page
• IMPC: Clptm1 gene page

Mutation origin

• Mutant Cell Lines: EPD0395_5_A09, EPD0395_5_A10, EPD0395_5_A12, EPD0395_5_B10, EPD0395_5_B12, EPD0395_5_C10, EPD0395_5_C11, EPD0395_5_C12, EPD0395_5_D10, EPD0395_5_E09, EPD0395_5_E11, EPD0395_5_E12, EPD0395_5_F09, EPD0395_5_F10, EPD0395_5_F12, EPD0395_5_G09, EPD0395_5_G10, EPD0395_5_G11, EPD0395_5_H09, EPD0395_5_H10, EPD0395_5_H11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 19372598 of Chromosome 7 upstream of the critical exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 19373363. Exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Western blot analysis confirmed disruption of protein expression in brain tissue lysates from E18 homozygous embryos. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:357551)

Expression

In Mice Carrying this Mutation:	11 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Clptm1 Mutation: 35 strains or lines available

Notes

Cell line EPD0395_5_D10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:357551.

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)