Enpp2^tm1Mmas
Targeted Allele Detail

Summary

• Symbol: Enpp2^tm1Mmas
• Name: ectonucleotide pyrophosphatase/phosphodiesterase 2; targeted mutation 1, Masayuki Masu
• MGI ID: MGI:4418729
• Synonyms: ATX-, Enpp2^lacZ
• Gene: Enpp2 Location: Chr15:54702297-54816284 bp, - strand Genetic Position: Chr15, 21.6 cM, cytoband D2
• Alliance: Enpp2^tm1Mmas page

Small size, absence of embryo turning and angiogenic defects in the yolk sack of Enpp2^tm1Mmas/Enpp2^tm1Mmas embryos

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156013
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: Exons 6 and 7 were replaced with an IRES-lacZ and inverted neo cassette via homologous recombination. RT-PCR and western blot analysis confirmed the absence of mRNA and protein expression in homozygous embryos and extraembryonic coelomic fluid, respectively. (J:156013)

Generation of the Enpp2^tm1Mmas allele

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Enpp2 Mutation: 78 strains or lines available

References

• Original: J:156013 Koike S, et al., Autotaxin/lysophospholipase D-mediated lysophosphatidic acid signaling is required to form distinctive large lysosomes in the visceral endoderm cells of the mouse yolk sac. J Biol Chem. 2009 Nov 27;284(48):33561-70
• All: 5 reference(s)