Tg(Pgk1-flpo)10Sykr
Transgene Detail

Summary

• Symbol: Tg(Pgk1-flpo)10Sykr
• Name: transgene insertion 10, Shoshana Yakar
• MGI ID: MGI:4415609
• Synonyms: FLPo-10
• Transgene: Tg(Pgk1-flpo)10Sykr Location: unknown
• Alliance: Tg(Pgk1-flpo)10Sykr page

Transgene origin

• Strain of Origin: C57BL/6 x C3H

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Pgk1-flpo)10Sykr expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Pgk1

• Mutation details: A transgenic construct was created with the Pgk1 promoter driving mouse codon-optimized FLP (FLPo) recombinase. The linearized construct was injected into fertilized C57BL/6 x C3H oocytes. Six founder mice showing germline transmission were generated. These animals demostrated FLP expression as early as 3.5 embryonic days and adults displayed ubiquitious expression in all tissues examined with strongest expression in the testis and ovary. Line 10 showed 100% recombination efficiency when crossed to mice expressing a Ghr allele with a FRT-flanked PGK-Neo cassette in intron 5. (This Ghr allele is the parent construct of the Ghr^tm1.1Masp allele.) (J:154848, J:240544)

Schematic of the Tg(Pgk1-flpo)10Sykr transgene

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pgk1 (species not specified) Summary of all recombinase alleles driven by Pgk1.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:154848 Wu Y, et al., High-efficient FLPo deleter mice in C57BL/6J background. PLoS One. 2009;4(11):e8054
• All: 95 reference(s)