Nkain4^{Gt(M076C04)Vmel}
Gene trapped Allele Detail

Summary

• Symbol: Nkain4^{Gt(M076C04)Vmel}
• Name: Na+/K+ transporting ATPase interacting 4; gene trap M076C04, Harald von Melchner
• MGI ID: MGI:4413343
• Gene: Nkain4 Location: Chr2:180576565-180596492 bp, - strand Genetic Position: Chr2, 103.53 cM, cytoband H4
• Alliance: Nkain4^{Gt(M076C04)Vmel} page

Mutation origin

• Mutant Cell Line: M076C04
• Germline Transmission: Earliest citation of germline transmission: J:154726
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: U3CEO Vector Type: secretory trap
• Mutation details: The first intron of the endogenous gene is interrupted by the U3Ceo secretory gene trap vector. This vector comprises an enhancerless, promoterless Moloney mouse leukemia (retro)virus genome whose LTR U3 region contains the Ceo fusion gene, which encodes a protein joining the promoterless type II transmembrane and extracellular domains of human CD2 to bacterial neomycin phosphotransferase; the CD2-encoding DNA segment includes a cryptic splice acceptor site. Expression of the endogenous gene is undetectable by northern blot analysis of total brain RNA from homozygous mutant mice. (J:154726)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nkain4 Mutation: 21 strains or lines available

References

• Original: J:154726 De-Zolt S, et al., High-throughput trapping of secretory pathway genes in mouse embryonic stem cells. Nucleic Acids Res. 2006;34(3):e25
• All: 2 reference(s)