Tg(CD19-cre/ERT2)1Jsv
Transgene Detail

Summary

• Symbol: Tg(CD19-cre/ERT2)1Jsv
• Name: transgene insertion 1, J Sjef Verbeek
• MGI ID: MGI:4399751
• Synonyms: LC-1-hCD19-CreER^T2, Tg(CD19-cre/ERT2)1Sjv
• Transgene: Tg(CD19-cre/ERT2)1Jsv Location: unknown
• Alliance: Tg(CD19-cre/ERT2)1Jsv page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(CD19-cre/ERT2)1Jsv expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  CD19 (930)

  Cd19	(MGI:88319)

• Mutation details: A DNA fragment encoding cre/ERT2 (cre recombinase fused to the human estrogen receptor with a mutated ligand-binding domain) was inserted downstream of a 6.3-kb fragment of the human CD19 promoter. This construct was inserted into the LC-1 BAC via Red-mediated homologous recombination in E. coli. The recombinant BAC was injected into the pronuclei of fertilized C57BL/6 oocytes. Two founders were generated and one line designated LC-1-hCD19-CreER^T2 provided functional cre-mediated recombination. PCR analysis showed that animals carried a single copy of the transgene. (J:154796)

Generation of the Tg(CD19-cre/ERT2)1Jsv transgene

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CD19 (human) Summary of all recombinase alleles driven by CD19. Alliance human CD19 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:154796 Boross P, et al., Highly B lymphocyte-specific tamoxifen inducible transgene expression of CreER T2 by using the LC-1 locus BAC vector. Genesis. 2009 Nov;47(11):729-35
• All: 2 reference(s)