Vamp5^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Vamp5^{tm1(KOMP)Vlcg}
• Name: vesicle-associated membrane protein 5; targeted mutation 1, Velocigene
• MGI ID: MGI:4399506
• Gene: Vamp5 Location: Chr6:72345777-72357451 bp, - strand Genetic Position: Chr6, 32.27 cM, cytoband C3
• Alliance: Vamp5^{tm1(KOMP)Vlcg} page
• IMPC: Vamp5 gene page

Mutation origin

• Mutant Cell Lines: 13123A-E2, 13123A-E5, 13123A-F3, 13123A-G9
• Germline Transmission: Earliest citation of germline transmission: J:261567
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 11129bp between positions 72346235-72357363 of Chromosome 6 (Build GRCm39). All exons except the start codon were deleted and replaced with reporter genes. Western blot analysis confirmed the absence of encoded protein in the kidney and bladder of adult homozygous mutant mice. (J:136110, J:261567)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Vamp5 Mutation: 17 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 2 reference(s)