Ccn5^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Ccn5^{tm1(KOMP)Vlcg}
• Name: cellular communication network factor 5; targeted mutation 1, Velocigene
• MGI ID: MGI:4399364
• Synonyms: Ccn5^LacZ, Wisp2^{tm1(KOMP)Vlcg}
• Gene: Ccn5 Location: Chr2:163662781-163675066 bp, + strand Genetic Position: Chr2, 84.49 cM
• Alliance: Ccn5^{tm1(KOMP)Vlcg} page
• IMPC: Ccn5 gene page

Mutation origin

• Mutant Cell Lines: 12404A-A3, 12404A-D2, 12404A-G4
• Germline Transmission: Earliest citation of germline transmission: J:260675
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 11155bp between positions 163663245-163674399 of Chromosome 2 (Build GRCm39). Exons 2-5, encoding all of the conserved protein coding regions, are replaced by a lacZ cassette. qPCR analysis confirmed the absence of mRNA expression in the tibia of homozygous mutant mice. (J:136110, J:260675)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Ccn5 Mutation: 25 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 3 reference(s)