Muc1^{tm1.1(cre/ERT2)Lcm}
Targeted Allele Detail

Summary

• Symbol: Muc1^{tm1.1(cre/ERT2)Lcm}
• Name: mucin 1, transmembrane; targeted mutation 1.1, Lewis Murtaugh
• MGI ID: MGI:4399063
• Synonyms: Muc1^IC2, Muc1^IRES-CreERT2
• Gene: Muc1 Location: Chr3:89136364-89140688 bp, + strand Genetic Position: Chr3, 39.02 cM
• Alliance: Muc1^{tm1.1(cre/ERT2)Lcm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159445
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Muc1^{tm1.1(cre/ERT2)Lcm} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Muc1 (MGI:97231)

• Mutation details: This allele was derived by crossing mice bearing Muc^{tm1(cre/ERT2)Lcm} to Gt(ROSA)26Sor^tm1(FLP1)Dym mice to delete the frt site-flanked neomycin resistance cassette in the germline. The present, neo^-, allele contains, downstream of the termination codon of the endogenous gene, an internal ribosomal entry site (IRES) followed by DNA encoding Cre recombinase joined to ERT2 and a single frt site (IRES-CreERT2-FRT). ERT2 is a triply mutated (G400V, M543A, L544A) human estrogen receptor ligand-binding domain (LBD) that renders the fusion protein highly sensitive to activation by 4-hydroxytamoxifen, but unresponsive to the receptor's native ligands. The cre/ERT2 fusion protein is expressed under control of the endogenous promoter without disrupting expression of the endogenous gene. Tamoxifen-induced Cre activity has been demonstrated in acinar and ductal cells of the adult pancreas and in their embryonic precursors. (J:159445)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Muc1 (mouse) Summary of all recombinase alleles driven by Muc1. Alliance mouse Muc1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Muc1 Mutation: 35 strains or lines available

References

• Original: J:159445 Kopinke D, et al., Exocrine-to-endocrine differentiation is detectable only prior to birth in the uninjured mouse pancreas. BMC Dev Biol. 2010;10:38
• All: 2 reference(s)