Tg(BEST1-rtTA,tetO-cre)1Yzl
Transgene Detail

Summary

• Symbol: Tg(BEST1-rtTA,tetO-cre)1Yzl
• Name: transgene insertion 1, Yun-Zheng Le
• MGI ID: MGI:4398912
• Synonyms: PVMD2-rtTA::tetO-PhCMVcre, VMD2-cre
• Transgene: Tg(BEST1-rtTA,tetO-cre)1Yzl Location: unknown
• Alliance: Tg(BEST1-rtTA,tetO-cre)1Yzl page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(BEST1-rtTA,tetO-cre)1Yzl expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  BEST1 (7439)

  Best1

  (MGI:1346332)

• Mutation details: Two transgenic fragments were co-injected to generate the mouse line. One fragment contains a 3 kb human BEST1 promoter fragment driving expression of the tetracycline-inducible transactivator rtTA and the SV40 polyA sequence. The second fragment consists of the tetracycline-responsive regulatory element (tetO) and human cytomegalovirus immediate early enhancer (hCMV) controlling expression of a translationally optimized cre sequence followed by the mouse metallothionein (Mt1) polyadenylation signal. After co-injection into zygotes, 10 founders were obtained with co-integration of both fragments into a single chromosome being observed in all founders. One line demonstrating substantial activity in Muller glial cells was characterized further. (J:154799)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	BEST1 (human) Summary of all recombinase alleles driven by BEST1. Comparative matrix view of recombinase activities Alliance human BEST1 gene page

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:132948 Le YZ, et al., Inducible expression of cre recombinase in the retinal pigmented epithelium. Invest Ophthalmol Vis Sci. 2008 Mar;49(3):1248-53
• All: 43 reference(s)