Gtsf1^tm1Miya
Targeted Allele Detail

Summary

• Symbol: Gtsf1^tm1Miya
• Name: gametocyte specific factor 1; targeted mutation 1, Jun-ichi Miyazaki
• MGI ID: MGI:4397586
• Gene: Gtsf1 Location: Chr15:103310885-103338872 bp, - strand Genetic Position: Chr15, 58.93 cM, cytoband F3
• Alliance: Gtsf1^tm1Miya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154374
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 through 4 were replaced with an IRES-beta-geo and puro cassette. The absence of protein expression was confirmed by western blot analysis on week 7 testis extracts. (J:154374)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gtsf1 Mutation: 19 strains or lines available

Notes

ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:154374 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

References

• Original: J:154374 Yoshimura T, et al., Gtsf1/Cue110, a gene encoding a protein with two copies of a CHHC Zn-finger motif, is involved in spermatogenesis and retrotransposon suppression in murine testes. Dev Biol. 2009 Nov 1;335(1):216-27
• All: 2 reference(s)