Id1^tm3Bene
Targeted Allele Detail

Summary

• Symbol: Id1^tm3Bene
• Name: inhibitor of DNA binding 1, HLH protein; targeted mutation 3, Robert Benezra
• MGI ID: MGI:4366910
• Synonyms: Id1^floxed, Id1^L
• Gene: Id1 Location: Chr2:152578171-152579330 bp, + strand Genetic Position: Chr2, 75.41 cM
• Alliance: Id1^tm3Bene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154023
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An FRT-neo-FRT cassette followed by a loxP site was inserted after the coding region of Id1. Another loxP was inserted before the Id1 gene such that the entire locus was floxed. This allele was generated by homologous recombination in Bruce4 ES cells. Southern blot positive ESC clones were injected into non-isogenic FVB blastocyts. Positive male chimeras were bred to B6J females. Black pups were screened by PCR genotyping and bred to B6 ACTB::FLPe mouse (Artemis Pharmaceuticals) to excise the neo cassette in vivo. (J:154023)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Id1 Mutation: 8 strains or lines available

References

• Original: J:154023 Nam HS, et al., High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell. 2009 Nov 6;5(5):515-26
• All: 11 reference(s)