Pfkfb2tm1e(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Pfkfb2tm1e(KOMP)Wtsi |
Name: |
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2; targeted mutation 1e, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:4364707 |
Gene: |
Pfkfb2 Location: Chr1:130616919-130656990 bp, - strand Genetic Position: Chr1, 56.89 cM, cytoband E4
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IMPC: |
Pfkfb2 gene page |
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Mutant Cell Lines: |
EPD0308_4_B06, EPD0308_4_F05, EPD0308_4_H06 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A1.N3 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 130635655 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:148605, J:173534)
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Original: |
J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009; |
All: |
2 reference(s) |
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