Usp22^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Usp22^{tm1a(KOMP)Wtsi}
• Name: ubiquitin specific peptidase 22; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4364127
• Synonyms: Usp22^lacZ
• Gene: Usp22 Location: Chr11:61042611-61065881 bp, - strand Genetic Position: Chr11, 37.96 cM
• Alliance: Usp22^{tm1a(KOMP)Wtsi} page
• IMPC: Usp22 gene page

Mutation origin

• Mutant Cell Lines: EPD0119_2_A09, EPD0119_2_D11, EPD0171_3_D04, EPD0171_3_D08, EPD0171_3_H03
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 61058865 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 61059653. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
  
  Special note about mutant cell line EPD0119_2_D11 only: The mutation generated in this ES cell line results in a hypomorphic allele. qRT-PCR analysis revealed a minimal level (~3%) of residual expression of correctly spliced mRNA in brain and small intestine tissues isolated from adult homozygous mutant mice. Western blot analysis confirmed a significant but incomplete reduction of protein levels in both tissues (J:233975). Other cell lines associated with this allele have not been tested. (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Usp22 Mutation: 30 strains or lines available

Notes

Special note about mutant cell line EPD0119_2_D11 only: The mutation generated in this ES cell line results in a hypomorphic allele. qRT-PCR analysis revealed a minimal level (~3%) of residual expression of correctly spliced mRNA in brain and small intestine tissues isolated from adult homozygous mutant mice. Western blot analysis confirmed a significant but incomplete reduction of protein levels in both tissues (J:233975). Other cell lines associated with this allele have not been tested.

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 13 reference(s)