Zfp316tm1a(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Zfp316tm1a(KOMP)Wtsi |
Name: |
zinc finger protein 316; targeted mutation 1a, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:4363490 |
Gene: |
Zfp316 Location: Chr5:143235452-143255777 bp, - strand Genetic Position: Chr5, 82.03 cM
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IMPC: |
Zfp316 gene page |
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Mutant Cell Lines: |
EPD0317_6_A02, EPD0317_6_B02, EPD0317_6_B04, EPD0317_6_C03, EPD0317_6_E03, EPD0317_6_G01, EPD0317_6_G02, EPD0317_6_H01, EPD0317_6_H03, EPD0317_6_H04 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A1.N3 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Pgk_P
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Mutation details: The L1L2_Pgk_P cassette was inserted at position 143248900 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 143249577. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:148605, J:173534)
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Original: |
J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009; |
All: |
2 reference(s) |
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