Dnase1l2^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dnase1l2^{tm1(KOMP)Wtsi}
• Name: deoxyribonuclease 1-like 2; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362265
• Gene: Dnase1l2 Location: Chr17:24659061-24662075 bp, - strand Genetic Position: Chr17, 12.39 cM, cytoband A3.3
• Alliance: Dnase1l2^{tm1(KOMP)Wtsi} page
• IMPC: Dnase1l2 gene page

Mutation origin

• Mutant Cell Lines: EPD0296_2_A10, EPD0296_2_B11, EPD0296_2_B12, EPD0296_2_C09, EPD0296_2_D10, EPD0296_2_F10, EPD0296_2_F11, EPD0296_2_G09, EPD0296_2_G11, EPD0296_2_G12
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 1406 starting at position 24660403 and ending at position 24661809 of Chromosome 17 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Expression

In Mice Carrying this Mutation:	172 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Dnase1l2 Mutation: 23 strains or lines available

Notes

Cell line EPD0296_2_A10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:200082

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 7 reference(s)