Esr1^tm2.1Gsc
Targeted Allele Detail

Summary

• Symbol: Esr1^tm2.1Gsc
• Name: estrogen receptor 1 (alpha); targeted mutation 2.1, Gunther Schutz
• MGI ID: MGI:4361324
• Synonyms: ERalpha^EAAE
• Gene: Esr1 Location: Chr10:4561989-4955633 bp, + strand Genetic Position: Chr10, 2.03 cM
• Alliance: Esr1^tm2.1Gsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152849
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Nucleotide substitutions resulted in amino acid substitutions of glutamic acid for tyrosine at position 201 (Y201E), alanine for lysine at position 210 (K210A), alanine for lysine at position 214 (K214A), and glutamic acid for arginine at position 215 (R215E). A floxed neo cassette used for selection was removed by transient cre expression in ES cells. The mutated amino acids are a part of the DNA recognition helix. (J:152849)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Esr1 Mutation: 67 strains or lines available

References

• Original: J:152849 Ahlbory-Dieker DL, et al., DNA binding by estrogen receptor-alpha is essential for the transcriptional response to estrogen in the liver and the uterus. Mol Endocrinol. 2009 Oct;23(10):1544-55
• All: 4 reference(s)