Obscn^tm1Chen
Targeted Allele Detail

Summary

• Symbol: Obscn^tm1Chen
• Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF; targeted mutation 1, Ju Chen
• MGI ID: MGI:4361272
• Gene: Obscn Location: Chr11:58885082-59027201 bp, - strand Genetic Position: Chr11, 36.4 cM
• Alliance: Obscn^tm1Chen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153150
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was replaced with a floxed LacZ, GFP, and frt flanked neo cassette. The absence of expression of both the A and B form was confirmed by western blot analysis on heart, tibiallis anterior, and soleus extracts. (J:153150)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Obscn Mutation: 420 strains or lines available

References

• Original: J:153150 Lange S, et al., Obscurin determines the architecture of the longitudinal sarcoplasmic reticulum. J Cell Sci. 2009 Aug 1;122(Pt 15):2640-50
• All: 6 reference(s)