Dact1^tm1.1Bnrc
Targeted Allele Detail

Summary

• Symbol: Dact1^tm1.1Bnrc
• Name: dishevelled-binding antagonist of beta-catenin 1; targeted mutation 1.1, Benjamin N R Cheyette
• MGI ID: MGI:4360072
• Synonyms: Dact1^neodelta
• Gene: Dact1 Location: Chr12:71356658-71366881 bp, + strand Genetic Position: Chr12, 29.39 cM
• Alliance: Dact1^tm1.1Bnrc page

Spectrum of posterior defects in Sestd1^tm1.1Bnrc/Sestd1^tm1.1Bnrc and Dact1^tm1.1Bnrc/Dact1^tm1.1Bnrc mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152701
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette, followed by a loxP site, upstream of exon 2, and a second loxP site downstream of exon 6. Cre-mediated recombination then removed exon 2. Western blot analysis confirmed the absence of protein expression in uterine tissue from homozygous mice. (J:152701)

Expression

In Structures Affected by this Mutation:

21 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dact1 Mutation: 18 strains or lines available

References

• Original: J:152701 Suriben R, et al., Posterior malformations in Dact1 mutant mice arise through misregulated Vangl2 at the primitive streak. Nat Genet. 2009 Sep;41(9):977-85
• All: 4 reference(s)