Lat^tm7.1Mal
Targeted Allele Detail

Summary

• Symbol: Lat^tm7.1Mal
• Name: linker for activation of T cells; targeted mutation 7.1, Bernard Malissen
• MGI ID: MGI:4357981
• Synonyms: Lat^OST, Lat^tm7Mal
• Gene: Lat Location: Chr7:125962996-125968742 bp, - strand Genetic Position: Chr7, 69.01 cM
• Alliance: Lat^tm7.1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209882
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Epitope tag, Hypomorph)
• Mutation: Insertion
• Mutation details: Sequence for a Gly-Ser-Gly spacer, a OneSTrEPtag (OST), two STOP codons, a loxP site, the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, the cre recombinase gene, a neomycin resistance gene cassette, and a second loxP site, was inserted in-frame after sequence coding for at the 3 end of the gene coding sequence in exon 11. Cre-mediated self-excision removed the cre+neo cassette in the male germline. The fusion of the OST to the protein can be used in affinity purification. Protein reduction from this allele is reduced to 25% of wild-type gene expression. (J:209882)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lat Mutation: 11 strains or lines available

References

• Original: J:209882 Roncagalli R, et al., Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub. Nat Immunol. 2014 Apr;15(4):384-392
• All: 1 reference(s)