Slc4a1^tm1Phsl
Targeted Allele Detail

Summary

• Symbol: Slc4a1^tm1Phsl
• Name: solute carrier family 4 (anion exchanger), member 1; targeted mutation 1, Philip S Low
• MGI ID: MGI:4357799
• Synonyms: band-3^-
• Gene: Slc4a1 Location: Chr11:102239646-102256107 bp, - strand Genetic Position: Chr11, 66.29 cM, cytoband D
• Alliance: Slc4a1^tm1Phsl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152245
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: 33 base-pairs that encode a beta-hairpin loop were deleted from exon 7. A floxed neomycin cassette was included downstream of exon 4 for targeting purposes. Correct targeting was confirmed by genomic PCR. RNA Expression of the locus was 8% of normal in homozgyote bone marrow. The 11-amino acid beta-hairpin loop in the cytoplasmic domain of the protein product is responsible for ankyrin binding in erythrocytes. (J:152245)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc4a1 Mutation: 54 strains or lines available

References

• Original: J:152245 Anong WA, et al., Adducin forms a bridge between the erythrocyte membrane and its cytoskeleton and regulates membrane cohesion. Blood. 2009 Aug 27;114(9):1904-12
• All: 1 reference(s)